Accelerate PhenoTest uses which technique?

Accelerate PhenoTest uses which technique?

• A. FISH
• B. Nucleic acid extraction
• C. MALDI-TOF
• D. PCR amplification

Answer: (A)

Accelerate PhenoTest identifies organisms using fluorescence in situ hybridization. FISH uses fluorescently labeled probes that bind to ribosomal RNA sequences specific to particular organisms, allowing rapid visualization of the organism directly in cells and enabling quick identification from positive culture broths. This approach is different from MALDI-TOF, which relies on protein mass spectra, or PCR-based methods that amplify nucleic acids, and it doesn’t depend on a separate nucleic acid extraction step. The PhenoTest platform then pairs this rapid ID with its growth-based antimicrobial susceptibility testing, but the identification technique itself is FISH.

Accelerate PhenoTest identifies organisms using fluorescence in situ hybridization. FISH uses fluorescently labeled probes that bind to ribosomal RNA sequences specific to particular organisms, allowing rapid visualization of the organism directly in cells and enabling quick identification from positive culture broths. This approach is different from MALDI-TOF, which relies on protein mass spectra, or PCR-based methods that amplify nucleic acids, and it doesn’t depend on a separate nucleic acid extraction step. The PhenoTest platform then pairs this rapid ID with its growth-based antimicrobial susceptibility testing, but the identification technique itself is FISH.